Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1984-6-5
pubmed:abstractText
Oxytricha nova is a hypotrichous ciliate containing a transcriptionally active macronucleus and a transcriptionally inactive micronucleus. Two-dimensional gel electrophoresis shows that macronuclei contain a normal complement of inner histones. However, despite extensive efforts, no classical H1-like protein has been detected. Micrococcal nuclease digestion indicates a nucleosome repeat length of approximately 220 bp for macronuclear chromatin. Thermal denaturation profiles of macronuclear chromatin in 0.2 mM EDTA display four transitions at about 46, 57, 64, and 79 degrees C. The lowest of these shifts to higher temperature as the ionic strength is raised to 3-5 mM Na phosphate. These results are consistent with the absence of H1 and a nucleosome repeat of 220-230 bp. Circular dichroism (CD) results agree with these findings. By contrast, micronuclear chromatin displays a much smaller premelt and a more suppressed DNA CD signal at 285 nm, consistent with a micronuclear chromatin repeat of 165-185 bp as determined by micrococcal nuclease digestion.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/6718249-106365, http://linkedlifedata.com/resource/pubmed/commentcorrection/6718249-214760, http://linkedlifedata.com/resource/pubmed/commentcorrection/6718249-291904, http://linkedlifedata.com/resource/pubmed/commentcorrection/6718249-416437, http://linkedlifedata.com/resource/pubmed/commentcorrection/6718249-4209264, http://linkedlifedata.com/resource/pubmed/commentcorrection/6718249-450703, http://linkedlifedata.com/resource/pubmed/commentcorrection/6718249-4621095, http://linkedlifedata.com/resource/pubmed/commentcorrection/6718249-4628029, http://linkedlifedata.com/resource/pubmed/commentcorrection/6718249-489588, http://linkedlifedata.com/resource/pubmed/commentcorrection/6718249-493153, http://linkedlifedata.com/resource/pubmed/commentcorrection/6718249-508905, http://linkedlifedata.com/resource/pubmed/commentcorrection/6718249-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/6718249-5778650, http://linkedlifedata.com/resource/pubmed/commentcorrection/6718249-643604, http://linkedlifedata.com/resource/pubmed/commentcorrection/6718249-6771110, http://linkedlifedata.com/resource/pubmed/commentcorrection/6718249-7265232, http://linkedlifedata.com/resource/pubmed/commentcorrection/6718249-7328651, http://linkedlifedata.com/resource/pubmed/commentcorrection/6718249-7356945, http://linkedlifedata.com/resource/pubmed/commentcorrection/6718249-7396522, http://linkedlifedata.com/resource/pubmed/commentcorrection/6718249-7433093, http://linkedlifedata.com/resource/pubmed/commentcorrection/6718249-7443530, http://linkedlifedata.com/resource/pubmed/commentcorrection/6718249-98283
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3201-17
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Physical structure of gene-sized chromatin from the protozoan Oxytricha.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't