Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 4
pubmed:dateCreated
1984-4-19
pubmed:abstractText
The oxygen plasma etching technique of Humphreys et al. has been modified and applied for use on the entire block face of methacrylate and epoxy embedded biological specimens. This technique greatly improves the visualization of tissue and cellular components, including intracellular organelles. This procedure, when coupled with the use of a miniature microtome which functions inside the SEM specimen chamber, greatly increases the usefulness of serial SEM images instead of serial sections for 3-dimensional reconstructions. Although this technique is demonstrated using the central nervous system of the nudibranch mollusc Hermissenda crassicornis and the squid (Loligo pealei) fin nerve on both osmicated and non-osmicated tissue, it should have broad applicability to most biological material.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0586-5581
pubmed:author
pubmed:issnType
Print
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1877-85
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed:year
1983
pubmed:articleTitle
Oxygen plasma etching of entire block faces improves the resolution and usefulness of serial scanning electron microscopic images.
pubmed:publicationType
Journal Article