Linked Life Data

6548709

Source:http://linkedlifedata.com/resource/pubmed/id/6548709

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1985-1-23
pubmed:abstractText
The early-methionine-labelled (Em) polypeptide is the most abundant single polypeptide found in the cytosolic fraction (30 000 X g supernatant) of dry wheat embryos. Synthesis of this polypeptide is readily detectable during the earliest stages (0-3 h) of wheat embryo germination, by labelling with [35S]methionine. Thereafter, synthesis of the Em polypeptide declines rapidly. A DNA sequence encoding a portion of this polypeptide has been isolated by the molecular cloning of DNA complementary to the messenger RNA from dry wheat embryos. The identity of this cloned sequence has been confirmed by hybrid-selected translation of the Em messenger, the product being analysed by one- and two-dimensional polyacrylamide gel electrophoresis, and by analysis of its partial proteolytic digestion products. Gel blot hybridisation of RNA isolated from embryos at successive stages of germination with this cloned DNA sequence confirms that the decline in the synthesis of the Em polypeptide, detected by labelling in vivo, is a consequence of the degradation of its corresponding messenger RNA.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0014-2956
pubmed:author
pubmed:issnType
Print
pubmed:day
3
pubmed:volume
145
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
351-7
pubmed:dateRevised
2007-7-23
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Developmental regulation of gene expression in wheat embryos. Molecular cloning of a DNA sequence encoding the early-methionine-labelled (Em) polypeptide.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't