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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1984-3-22
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pubmed:abstractText |
Monoclonal antibodies against amyloid fibril protein AA were produced by cell fusion of murine P3 X 63-Ag8.653 myeloma cells with spleen cells of immunized Balb/c mice. To increase immunogenicity, protein AA was coupled to horseradish peroxidase (HRP) or human high molecular weight kininogen (HMWK). Using micro-ELISA (enzyme-linked immunosorbent essay) seven hybridoma cell lines secreting antibodies that specifically bind to protein AA have been selected and cloned. When applied to formalin-fixed paraffin sections of a variety of different amyloid types using immunoperoxidase methods, five monoclonal antibodies bound specifically and strongly to amyloid only of the AA type. Since a series of different AA-amyloids could be stained, these reagents may be used to routinely diagnose AA-amyloidosis in tissue sections. A monoclonal antibody against HRP has also been produced that has been utilized to develop a monoclonal peroxidase-antiperoxidases (PAP) complex. When three immunoperoxidase methods were compared, the sensitivity of a conventional rat PAP was comparable to the monoclonal PAP complex, but the latter was easier to handle. Both methods were more sensitive than the indirect immunoperoxidase technique.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amyloid,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Horseradish Peroxidase,
http://linkedlifedata.com/resource/pubmed/chemical/Kininogens,
http://linkedlifedata.com/resource/pubmed/chemical/Serum Amyloid A Protein
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0022-1554
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
32
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
322-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:6363521-Amyloid,
pubmed-meshheading:6363521-Amyloidosis,
pubmed-meshheading:6363521-Antibodies, Monoclonal,
pubmed-meshheading:6363521-Antibody Specificity,
pubmed-meshheading:6363521-Horseradish Peroxidase,
pubmed-meshheading:6363521-Humans,
pubmed-meshheading:6363521-Hybridomas,
pubmed-meshheading:6363521-Immunization,
pubmed-meshheading:6363521-Immunochemistry,
pubmed-meshheading:6363521-Immunoenzyme Techniques,
pubmed-meshheading:6363521-Kininogens,
pubmed-meshheading:6363521-Multiple Myeloma,
pubmed-meshheading:6363521-Serum Amyloid A Protein,
pubmed-meshheading:6363521-Spleen
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pubmed:year |
1984
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pubmed:articleTitle |
Monoclonal antibodies against amyloid fibril protein AA. Production, specificity, and use for immunohistochemical localization and classification of AA-type amyloidosis.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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