rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1983-10-28
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pubmed:databankReference |
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pubmed:abstractText |
We determined the nucleotide sequence of a DNA fragment containing the ftsI gene coding for the penicillin-binding protein 3 (PBP-3), an indispensable enzyme for cell division of Escherichia coli. The entire ftsI gene was within the 2.8 kilobase PvuII fragment derived from the chromosomal segment on pLC26-6 (Nishimura et al. 1977). The coding region for PBP-3 was identified by comparison with the N-terminal amino acid sequence of in vitro synthesized PBP-3. The structural gene for ftsI consisted of 1,764 base-pairs coding for a 588 amino acid residue-polypeptide with a molecular weight of 63,850. PBP-3 synthesized in vitro showed a lower mobility in SDS-gel electrophoresis than that of the authentic PBP-3, suggesting that the primary translation product of the ftsI gene may be processed to yield mature PBP-3.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/FtsI protein, E coli,
http://linkedlifedata.com/resource/pubmed/chemical/Hexosyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Muramoylpentapeptide...,
http://linkedlifedata.com/resource/pubmed/chemical/Penicillin-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Peptidoglycan Glycosyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/Peptidyl Transferases
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pubmed:status |
MEDLINE
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pubmed:issn |
0026-8925
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:volume |
191
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:6350821-Bacterial Proteins,
pubmed-meshheading:6350821-Base Sequence,
pubmed-meshheading:6350821-Carrier Proteins,
pubmed-meshheading:6350821-Cell Division,
pubmed-meshheading:6350821-DNA, Bacterial,
pubmed-meshheading:6350821-Escherichia coli,
pubmed-meshheading:6350821-Escherichia coli Proteins,
pubmed-meshheading:6350821-Genes,
pubmed-meshheading:6350821-Genes, Bacterial,
pubmed-meshheading:6350821-Hexosyltransferases,
pubmed-meshheading:6350821-Molecular Weight,
pubmed-meshheading:6350821-Muramoylpentapeptide Carboxypeptidase,
pubmed-meshheading:6350821-Penicillin-Binding Proteins,
pubmed-meshheading:6350821-Peptidoglycan Glycosyltransferase,
pubmed-meshheading:6350821-Peptidyl Transferases
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pubmed:year |
1983
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pubmed:articleTitle |
On the process of cellular division in Escherichia coli: nucleotide sequence of the gene for penicillin-binding protein 3.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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