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pubmed:abstractText |
Phytase has been purified from intestinal mucosa of rats fed on a phytic acid-enriched diet. The purification consisted of four steps: homogenate preparation, ultrafiltration procedure, DEAE cellulose chromatography, and Sephadex G-100 chromatography. The enzyme was purified 828-fold, as can be seen from the polyacrylamide gel electrophoresis pattern.
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