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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1984-3-29
|
| pubmed:abstractText |
The subunit stoichiometry of oligomeric enzymes can be determined by immuno-electron microscopy using monoclonal antibodies against the individual subunits. Monoclonal antibodies against native F1-ATPase of Escherichia coli were prepared that were specific for the alpha-subunit. The immune complexes of F1 and monoclonal antibodies were isolated. Electron microscopy revealed the presence of three immunoglobulins per molecule of F1-ATPase. This unequivocally demonstrates an alpha 3 stoichiometry for the F1-ATPase of E. coli.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0022-2836
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
173
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
131-6
|
| pubmed:dateRevised |
2001-11-2
|
| pubmed:meshHeading |
pubmed-meshheading:6230457-Antibodies, Monoclonal,
pubmed-meshheading:6230457-Antigen-Antibody Complex,
pubmed-meshheading:6230457-Chromatography, Gel,
pubmed-meshheading:6230457-Escherichia coli,
pubmed-meshheading:6230457-Microscopy, Electron,
pubmed-meshheading:6230457-Proton-Translocating ATPases
|
| pubmed:year |
1984
|
| pubmed:articleTitle |
Use of monoclonal antibodies in immuno-electron microscopy for the determination of subunit stoichiometry in oligomeric enzymes. There are three alpha-subunits in the F1-ATPase of Escherichia coli.
|
| pubmed:publicationType |
Journal Article
|