Linked Life Data

6205014

Source:http://linkedlifedata.com/resource/pubmed/id/6205014

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1984-9-12
pubmed:abstractText
A procedure is described for spectrophotometrically measuring the position and intensity of ELISA-stained protein bands after western blot analyses. Partially purified virus proteins separated by acrylamide gel electrophoresis and electrophoretically transblotted onto nitrocellulose paper were identified by ELISA reactions according to established procedures. The nitrocellulose paper strips were then rendered transparent on glass slides by treatment with a plastics solvent (Gelman Sepra-Clear). The slides were then scanned at 480 nm to obtain the precise migration distance and area under the curve (i.e., quantity) of each band, thus allowing accurate comparison of the proteins from different virus strains.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0166-0934
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
265-8
pubmed:dateRevised
2000-12-18
pubmed:meshHeading
pubmed:year
1984
pubmed:articleTitle
Spectrophotometric quantitation of peroxidase-stained protein bands following gel electrophoresis and the western blot transfer technique with respiratory syncytial virus.
pubmed:publicationType
Journal Article