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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1983-4-7
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pubmed:abstractText |
The solid-phase immunoassay for quantification of substance P has been developed. The assay is based on the repartition of anti-substance P antibodies between the insoluble phase-immobilized substance P and the free peptide. The immobilized substance P-antibody complex is then quantified with 125I-protein A. The method allowed detection of 10 pg of substance P. The values of substance P concentration obtained by the present method in different regions of the rat brain were comparable to those obtained by standard radioimmunoassay with 125I-tyr-8-substance P as tracer. The described solid-phase radioimmunoassay is a simple, sensitive, and reliable technique for quantification of substance P-like immunoreactivity in biological samples.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0022-3042
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
40
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
783-9
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:6186769-Animals,
pubmed-meshheading:6186769-Antigen-Antibody Complex,
pubmed-meshheading:6186769-Brain Chemistry,
pubmed-meshheading:6186769-Cross Reactions,
pubmed-meshheading:6186769-Kinetics,
pubmed-meshheading:6186769-Rabbits,
pubmed-meshheading:6186769-Radioimmunoassay,
pubmed-meshheading:6186769-Substance P
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pubmed:year |
1983
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pubmed:articleTitle |
Solid-phase radioimmunoassay for substance P.
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pubmed:publicationType |
Journal Article
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