Linked Life Data

6172310

Source:http://linkedlifedata.com/resource/pubmed/id/6172310

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1982-3-22
pubmed:abstractText
Experimental systems for the detection of somatic cell mutation to hypoxanthine-guanine phosphoribosyl transferase (HGPRT) deficiency in human lymphoma cells were developed. The cell line used was the Raji line established from Burkitt's lymphoma. The cloning efficiency (CE) in soft agar of the cells was enhanced approximately 5-fold by addition of 2 x 10(-4) to 4 x 10(-4)M dithiothreitol (DDT) and 1% human serum, as compared with the CE in control medium. Other thiols such a mercaptoethanol and thioglycerin were also able to increase the CE but not as efficiently as DTT. Under these culture conditions, 10(6) cells per 55-mm dish could be seeded into soft agar medium containing 5 micrograms/ml 6-thioguanine (6-TG) to detect a forward mutation without any metabolic cooperation. The optimum expression time was determined by treating the cells with 125 micrograms/ml ethyl methanesulfonate (EMS) for 20 hr at 37 degrees. A significant increase of mutation frequency was observed approximately 10 days after the 6-TG treatment, by which time the cells had gone through about 10 population doublings. After determining these experimental conditions, a comparison of mutation frequency among three mutagens, EMS, N-methyl-N'-nitro-N-nitrosoguanidine and ICR-170, was carried out. ICr-170 was the most efficient mutagen among these chemicals.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0016-450X
pubmed:author
pubmed:issnType
Print
pubmed:volume
72
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
459-63
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Culture conditions for a human lymphoma cell line (Raji) and their application to mutation research.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't