Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1981-8-27
pubmed:abstractText
Several species of anaerobic bacteria display variable Gram stain reactions which often make identification difficult. A simple, rapid method utilizing a 3% solution of potassium hydroxide to distinguish between gram-positive and gram-negative bacterial was tested on 213 strains of anaerobic bacteria representing 19 genera. The Gram stain reaction and KOH test results were compared with the antibiotic disk susceptibilities (vancomycin and colistin) the preliminary grouping of anaerobic bacteria. All three procedures were in agreement for the majority of strains examined. Some strains of clostridia, eubacteria, and bifidobacteria stained gram negative or gram variable; the KOH and antibiotic disk susceptibility tests correctly classified these strains as gram-positive. The KOH test incorrectly grouped some strains of Bacteroides sp., Fusobacterium sp., Leptotrichia buccalis, and Veillonella parvula, but all Gram stain results for these strains were consistent for gram-negative bacteria. The KOH test is a useful supplement to the Gram stain and antibiotic disk susceptibility testing for the initial classification of anaerobic bacteria.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
444-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1981
pubmed:articleTitle
Rapid method that aids in distinguishing Gram-positive from Gram-negative anaerobic bacteria.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't