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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0010453,
umls-concept:C0204727,
umls-concept:C0205409,
umls-concept:C0229671,
umls-concept:C0332261,
umls-concept:C0449432,
umls-concept:C1179435,
umls-concept:C1524073,
umls-concept:C1548799,
umls-concept:C1705248
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pubmed:issue |
2
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pubmed:dateCreated |
1980-9-23
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pubmed:abstractText |
A foetal-calf-serum component has been isolated that stimulates cells in culture to adopt typical fibroblast morphology. This "spreading" factor is a glycoprotein with a mol. wt. of approx. 62000 and contains 12% carbohydrate; it has an isoelectric point of 4.8. The method described allows purification to homogeneity as judged by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and analytical isoelectric focusing. Under cell-culture conditions the factor is active at 18 micrograms/ml.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6156670-1248519,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6156670-13575428,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6156670-14207225,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6156670-14240533,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6156670-14240539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6156670-14416837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6156670-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6156670-16590146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6156670-16747948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6156670-46112,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6156670-4859375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6156670-5242123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6156670-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6156670-5559963,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6156670-597302,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6156670-680013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6156670-923661,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6156670-92424
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
185
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
349-54
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:6156670-Amino Acids,
pubmed-meshheading:6156670-Animals,
pubmed-meshheading:6156670-Cattle,
pubmed-meshheading:6156670-Cell Line,
pubmed-meshheading:6156670-Cricetinae,
pubmed-meshheading:6156670-Culture Media,
pubmed-meshheading:6156670-Dialysis,
pubmed-meshheading:6156670-Dose-Response Relationship, Drug,
pubmed-meshheading:6156670-Electrophoresis,
pubmed-meshheading:6156670-Fetal Blood,
pubmed-meshheading:6156670-Fibroblasts,
pubmed-meshheading:6156670-Glycoproteins,
pubmed-meshheading:6156670-Isoelectric Focusing,
pubmed-meshheading:6156670-Kidney,
pubmed-meshheading:6156670-Vitronectin
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pubmed:year |
1980
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pubmed:articleTitle |
Isolation of a serum component that stimulates the spreading of cells in culture.
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pubmed:publicationType |
Journal Article
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