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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
1985-2-6
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pubmed:abstractText |
The effect of somatostatin (SRIF) analogs on the secretion of radioimmunoassayable SRIF (SRIF-LI) was studied in the model system of fetal rat hypothalamus cells maintained in long-term, primary dispersed culture. These experiments exploited the biologic activity of certain synthetic SRIFs which cross-react poorly in our standard RIA for SRIF. Carbachol-stimulated SRIF-LI secretion was suppressed by [DTrp8]-SRIF-14, [DTrp22]-SRIF-28 and the octapeptide, des-AA1,2,4,5,12,13[DTrp8]-SRIF. Effective concentrations of synthetic analogs were at or below 1 nM. des-Trp8-SRIF-14, an analog inactive in all other SRIF biological assays, was similarly ineffective in this assay system at doses up to 10 nM. Following removal of peptide by washing, peptide-treated and control cells responded similarly to depolarization with high potassium buffer. The data demonstrate a direct action of SRIF analogs on the hypothalamus to suppress SRIF-LI release and are consistent with the possibility that an endogenous peptide participates in 'ultrashortloop' negative feedback control of its own secretion in the intact animal.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Nov
|
pubmed:issn |
0028-3835
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
39
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
397-402
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:6151126-Animals,
pubmed-meshheading:6151126-Carbachol,
pubmed-meshheading:6151126-Cells, Cultured,
pubmed-meshheading:6151126-Dose-Response Relationship, Drug,
pubmed-meshheading:6151126-Female,
pubmed-meshheading:6151126-Hypothalamus,
pubmed-meshheading:6151126-Radioimmunoassay,
pubmed-meshheading:6151126-Rats,
pubmed-meshheading:6151126-Receptors, Muscarinic,
pubmed-meshheading:6151126-Somatostatin
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pubmed:year |
1984
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pubmed:articleTitle |
Somatostatin analogs inhibit somatostatin secretion from cultured hypothalamus cells.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|