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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1981-2-26
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pubmed:abstractText |
Cultured human fibroblasts degrade insulin by a receptor-mediated process. When intracellular hormone degradation is inhibited by chloroquine, 125I-labeled insulin internalizes and accumulates intracellularly. In contrast, cultured IM-9 lymphocytes do not degrade receptor-bound insulin or accumulate 125I-labeled insulin in the presence of chloroquine. Insulin-induced receptor loss occurs in both cell types, and chloroquine inhibits this process in fibroblasts but not in IM-9 lymphocytes. Transglutaminase is a membrane-associated enzyme thought to mediate the process of ligand-induced receptor aggregation and internalization; levels of this enzyme were high in fibroblasts but barely detectable in IM-9 lymphocytes. Furthermore, dansylcadaverine--a potent inhibitor of transglutaminase--blocked insulin-induced receptor loss in fibroblasts but was without effect in IM-9 lymphocytes. These results support the concept that insulin receptor regulation is mediated via an endocytotic internalization pathway in human fibroblasts and that the mechanisms of this process differ among cell types.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/6108561-15720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6108561-214784,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6108561-221835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6108561-230489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6108561-4342623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6108561-4359334,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6108561-437375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6108561-447843,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6108561-476501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6108561-479155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6108561-489568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6108561-489589,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6108561-5577450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6108561-6153122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6108561-6243633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6108561-659578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6108561-661952,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6108561-6991335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6108561-715440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6108561-744100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6108561-81488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/6108561-85262
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
77
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5975-8
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:6108561-Cadaverine,
pubmed-meshheading:6108561-Cell Transformation, Neoplastic,
pubmed-meshheading:6108561-Cells, Cultured,
pubmed-meshheading:6108561-Chloroquine,
pubmed-meshheading:6108561-Endocytosis,
pubmed-meshheading:6108561-Fibroblasts,
pubmed-meshheading:6108561-Humans,
pubmed-meshheading:6108561-Insulin,
pubmed-meshheading:6108561-Lymphocytes,
pubmed-meshheading:6108561-Receptor, Insulin,
pubmed-meshheading:6108561-gamma-Glutamyltransferase
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pubmed:year |
1980
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pubmed:articleTitle |
Regulation of insulin receptors: evidence for involvement of an endocytotic internalization pathway.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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