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rdf:type |
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lifeskim:mentions |
|
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pubmed:issue |
837
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pubmed:dateCreated |
1968-8-8
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pubmed:abstractText |
Proinsulin in nearly homogeneous form has been isolated from a preparation of porcine insulin. A molecular weight close to 9100 was calculated from the amino acid composition and from sedimentation-equilibrium studies. Through the action of trypsin this single-chain protein is transformed to desalanine insulin by cleavage of a polypeptide chain connecting the carboxy-terminus of the B chain to the amino-terminus of the A chain of insulin. The amino acid sequence of this connecting peptide was found to be Arg-Arg-Glu-Ala-Gln-Asn-Pro-Gln-Ala-Gly-Ala-Val-Glu-Leu-Gly-Gly-Gly-Leu-Gly-Gly-Leu-Gln-Ala-Leu-Ala-Leu-Glu-Gly-Pro-Pro-Gln-Lys-Arg.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0036-8075
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
12
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pubmed:volume |
161
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
165-7
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:5657063-Amino Acid Sequence,
pubmed-meshheading:5657063-Animals,
pubmed-meshheading:5657063-Biological Assay,
pubmed-meshheading:5657063-Cellulose,
pubmed-meshheading:5657063-Chromatography, Ion Exchange,
pubmed-meshheading:5657063-Chymotrypsin,
pubmed-meshheading:5657063-Electrophoresis, Disc,
pubmed-meshheading:5657063-Hypoglycemia,
pubmed-meshheading:5657063-Insulin,
pubmed-meshheading:5657063-Molecular Weight,
pubmed-meshheading:5657063-Peptides,
pubmed-meshheading:5657063-Proteins,
pubmed-meshheading:5657063-Seizures,
pubmed-meshheading:5657063-Swine,
pubmed-meshheading:5657063-Trypsin
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pubmed:year |
1968
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pubmed:articleTitle |
Porcine proinsulin: characterization and amino acid sequence.
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pubmed:publicationType |
Journal Article
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