rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1968-5-23
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pubmed:abstractText |
The ADPglucose pyrophosphorylases of 7 plant-leaf tissues were partially purified and characterized. In all cases the enzymes showed stability to heat treatment at 65 degrees for 5 minutes in the presence of 0.02 m phosphate buffer, pH 7.0. The leaf ADPglucose pyrophosphorylases were activated 5 to 15-fold by 3-phosphoglycerate. Fructose-6-phosphate and fructose 1, 6-diphosphate stimulated ADPglucose pyrophosphorylase to lesser extents. The A(0.5) (conc of activator required to give 50% of the observed maximal activation) of 3-phosphoglycerate for the barley enzyme was 7 x 10(-6)m while for the sorghum enzyme it was 3.7 x 10(-4)m. Inorganic phosphate proved to be an effective inhibitor of ADPglucose synthesis. The I(0.5) (conc of inhibitor that gave 50% inhibition of activity for the various leaf enzymes varied from 2 x 10(-5)m (barley) to 1.9 x 10(-4)m (sorghum). This inhibition was reversed or antagonized by the activator 3-phosphoglycerate. These results form the basis for an hypothesis of the regulation of leaf starch biosynthesis.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/5644247-13760681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5644247-13936070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5644247-14061729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5644247-14269338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5644247-14275118,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5644247-14282015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5644247-14298833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5644247-14304834,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5644247-14490887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5644247-16656075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5644247-4292834,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5644247-4379647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5644247-4957920,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5644247-5833412,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5644247-5836525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5644247-5856636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5644247-5895754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5644247-5922972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5644247-5942950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5644247-5961861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5644247-5971771,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5644247-6052439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5644247-6057640
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0032-0889
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:volume |
43
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
417-27
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1968
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pubmed:articleTitle |
Regulation of starch biosynthesis in plant leaves: activation and inhibition of ADPglucose pyrophosphorylase.
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pubmed:publicationType |
Journal Article
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