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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1972-6-8
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pubmed:abstractText |
The subcellular distribution of either [(14)C] or [(3)H]3'-methylcholanthrene was studied in rat liver following a single intraperitoneal injection of the labelled hydrocarbon 10 hours previously.Adsorbed or non-covalently bound methylcholanthrene and its metabolic derivatives occurred in all cell fractions studied with the exception of purified cell walls. The highest specific activities (d.p.m./mg. protein) were found in washed mitochondria, microsomes and ribosome-free microsomal membranes.Covalent binding of methylcholanthrene and its metabolic derivatives to different cell fractions of rat liver occurs to a small extent and is considered not to be significant. The highest degree of binding occurs in washed mitochondria, microsomes, ribosome-free microsomal membranes and their constituent core proteins.Cell sap which contains non-covalently bound 3'-methylcholanthrene was fractionated into pH 5 enzyme and pH 5 supernatant fractions. The pH 5 enzyme fraction which possesses a high specific activity (d.p.m./mg. protein) was further fractionated with ammonium sulphate into three fractions. The 0-30% ammonium sulphate fraction had the highest specific activity.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/5144545-13042814,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5144545-14082346,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5144545-14187619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5144545-16749021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5144545-4311814,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5144545-4967807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5144545-5230138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5144545-5231592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5144545-5357016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5144545-5504343,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5144545-5797110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5144545-6021504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/5144545-6025253
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0007-0920
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
25
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
845-52
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pubmed:dateRevised |
2011-7-20
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pubmed:meshHeading |
pubmed-meshheading:5144545-Adsorption,
pubmed-meshheading:5144545-Ammonium Sulfate,
pubmed-meshheading:5144545-Animals,
pubmed-meshheading:5144545-Carbon Isotopes,
pubmed-meshheading:5144545-Cell Fractionation,
pubmed-meshheading:5144545-Cell Membrane,
pubmed-meshheading:5144545-Cell Nucleus,
pubmed-meshheading:5144545-Endoplasmic Reticulum,
pubmed-meshheading:5144545-Hydrogen-Ion Concentration,
pubmed-meshheading:5144545-Injections, Intraperitoneal,
pubmed-meshheading:5144545-Liver,
pubmed-meshheading:5144545-Male,
pubmed-meshheading:5144545-Membranes,
pubmed-meshheading:5144545-Methylcholanthrene,
pubmed-meshheading:5144545-Microsomes, Liver,
pubmed-meshheading:5144545-Mitochondria, Liver,
pubmed-meshheading:5144545-Protein Binding,
pubmed-meshheading:5144545-RNA,
pubmed-meshheading:5144545-Rats,
pubmed-meshheading:5144545-Ribosomes,
pubmed-meshheading:5144545-Tritium
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pubmed:year |
1971
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pubmed:articleTitle |
Studies on the binding and distribution of radioactively labelled 3'-methylcholanthrene in subcellular fractions of rat liver.
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pubmed:publicationType |
Journal Article
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