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pubmed:otherAbstract |
PIP: Treating immature female rats with estradiol sc, 1 mcg in saline or .1 mcg in oil for 2 days, induced a peroxidase in the uterus which converted carbon-14 labeled estradiol into water soluble products in vitro. Uterine extracts prepared by Klebanoff's method were incubated for 1 hour at 38 degrees C with labeled estradiol, hydrogen peroxide, dichlorophenol, and bovine serum albumin in sodium ponsphate buffer. After extracting the medium with ether, the radioactivity in both phases was determined and the steroids in the ether phase were identified by thin layer chromatography. .1 mcg estradiol injected in saline did not affect either yield of estrone or water soluble metabolites. .1 mcg estradiol given in oil or 1 mcg in saline increased both the yield of soluble metabolites over fourfold and the percent conversion of estradiol to estrone sevenfold (oil) and twofold (saline). Thus estradiol is metabolized in the uterus; this metabolism may be a local control for duration of estrogen action.
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