Linked Life Data

50397

Source:http://linkedlifedata.com/resource/pubmed/id/50397

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1975-11-8
pubmed:abstractText
A comparison was made by transmission electron microscopy of the microfilaments in the surface layers of the early embryos of Triturus alpestris and Xenopus laevis at stages of development up to neurulation. Actin-like filaments which bound heavy meromyosin (HMM) were found in cell extracts of all stages, but were comparatively rare in the newly fertilized egg. Ten nm microfilaments were present throughout development in Xenopus, and from the mid-neurula stage in Triturus. Both kinds of microfilament were located in the circumferences of superficial ectoderm cells at the level of the apical junctions, the 10 nm microfilaments in association with desmosomes which began to develop before gastrulation in Xenopus. The accumulations of microfilaments in the apical constrictions, which form in ectoderm cells of Triturus early gastrulae when dissociated in a calcium-free medium, suggest that they are contractile elements. In the absence of such accumulations in the cell apices, the reverse curling exhibited by Xenopus ectodermal explants is attributed rather to a separation of the cells' lateral borders. Cytochalasin B (5 mug/ml) caused ectodermal explants from the early gastrulae of both species to disaggregate. With the rupture of the apical junctions there was a disorganization of the associated microfilamentous layer.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-0752
pubmed:author
pubmed:issnType
Print
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
127-46
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1975
pubmed:articleTitle
Microfilaments in the external surface layer of the early amphibian embryo.
pubmed:publicationType
Journal Article, Comparative Study