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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1980-1-19
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pubmed:abstractText |
The kinetics of the inhibition of DNA polymerases-alpha and -beta from sea urchin embryos by pyridoxal 5-phosphate were studied. The inhibition of DNA polymerase-alpha activity by pyridoxal 5-phosphate was competitive with activated DNA but noncompetitive with each deoxynucleoside triphosphate. With poly(dC)-oligo(dG)12-18 as a template-primer, however, the inhibition of DNA polymerase-alpha was competitive with dGTP but noncompetitive with the template-primer. These results suggest that DNA polymerase-alpha interacts with activated DNA and poly(dC)-oligo(dG)12-18 in different ways. The inhibition of DNA polymerase-beta by pyridoxal 5-phosphate was competitive with deoxynucleoside triphosphate using activated DNA as a template-primer and noncompetitive with activated DNA. Using poly(rA)-oligo(dT)12-18 as a template-primer, DNA polymerase-beta activity yielded sigmoid curves against both dTTP and the template-primer concentrations and was inhibited by pyridoxal 5-phosphate noncompetitively with respect to both dTTP and the template-primer. These results indicate that the inhibitory mode of DNA polymerase-alpha by pyridoxal 5-phosphate is different from that of DNA polymerase-beta.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/503844-13802337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/503844-190232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/503844-205546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/503844-332062,
http://linkedlifedata.com/resource/pubmed/commentcorrection/503844-33710,
http://linkedlifedata.com/resource/pubmed/commentcorrection/503844-4859478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/503844-52361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/503844-569291,
http://linkedlifedata.com/resource/pubmed/commentcorrection/503844-599147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/503844-60130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/503844-673840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/503844-692726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/503844-704362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/503844-911834
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
727-34
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:503844-Animals,
pubmed-meshheading:503844-Binding, Competitive,
pubmed-meshheading:503844-DNA Polymerase I,
pubmed-meshheading:503844-DNA Polymerase II,
pubmed-meshheading:503844-DNA-Directed DNA Polymerase,
pubmed-meshheading:503844-Embryo, Nonmammalian,
pubmed-meshheading:503844-Female,
pubmed-meshheading:503844-Kinetics,
pubmed-meshheading:503844-Pyridoxal Phosphate,
pubmed-meshheading:503844-Sea Urchins,
pubmed-meshheading:503844-Structure-Activity Relationship
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pubmed:year |
1979
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pubmed:articleTitle |
The mode of inhibitory action by pyridoxal 5-phosphate on DNA polymerase-alpha and -beta.
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pubmed:publicationType |
Journal Article
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