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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3 Pt 1
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pubmed:dateCreated |
1986-4-11
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pubmed:abstractText |
Studies were performed to investigate the contribution of lipoprotein-associated cholesteryl ester (CE) in the monkey to circulating free cholesterol (FC). Monkey plasma was incubated with [14C]- or [3H]cholesteryl ester, and radiolabeled low-density lipoproteins (LDL) and high-density lipoproteins (HDL) were isolated by ultracentrifugation. Animals received labeled LDL or HDL. A rapid transfer of CE between lipoproteins was observed, consistent with an active CE transfer protein activity in the monkey. Within 4 h the percent of plasma radioactivity in FC after injection of CE-labeled LDL or HDL was, respectively, 30 and 7% of that of the ester. To determine whether the generation of FC was due to a circulating plasma cholesteryl ester hydrolase, monkey plasma was incubated with CE-labeled lipoproteins with and without 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB). A small amount of FC (less than 3% of the radioactivity) was generated during this incubation but most of the FC production was inhibited by DTNB. Although a small amount of FC can be produced by a plasma cholesteryl esterase (perhaps via reverse action of lecithin-cholesterol acyltransferase), most of the FC in plasma derived from lipoprotein-associated CE is probably due to tissue uptake of lipoproteins and subsequent intracellular hydrolysis of the CE to produce FC.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CETP protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cholesterol,
http://linkedlifedata.com/resource/pubmed/chemical/Cholesterol Ester Transfer Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cholesterol Esters,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoproteins, HDL,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoproteins, LDL,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylcholine-Sterol...,
http://linkedlifedata.com/resource/pubmed/chemical/Sterol Esterase,
http://linkedlifedata.com/resource/pubmed/chemical/lipoprotein cholesterol ester, human
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0002-9513
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
250
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
E265-8
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:3953812-Animals,
pubmed-meshheading:3953812-Carrier Proteins,
pubmed-meshheading:3953812-Cholesterol,
pubmed-meshheading:3953812-Cholesterol Ester Transfer Proteins,
pubmed-meshheading:3953812-Cholesterol Esters,
pubmed-meshheading:3953812-Glycoproteins,
pubmed-meshheading:3953812-Hydrolysis,
pubmed-meshheading:3953812-Lipoproteins,
pubmed-meshheading:3953812-Lipoproteins, HDL,
pubmed-meshheading:3953812-Lipoproteins, LDL,
pubmed-meshheading:3953812-Macaca mulatta,
pubmed-meshheading:3953812-Male,
pubmed-meshheading:3953812-Phosphatidylcholine-Sterol O-Acyltransferase,
pubmed-meshheading:3953812-Sterol Esterase
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pubmed:year |
1986
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pubmed:articleTitle |
Generation of plasma free cholesterol from circulating lipoprotein-associated cholesteryl ester.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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