rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
2
|
pubmed:dateCreated |
1986-4-4
|
pubmed:abstractText |
Addition of vasopressin (1 microM) to isolated rat hepatocytes prelabeled with [32P]phosphate was accompanied by a 250% increase in the phosphorylation of phospholipid methyltransferase. Vasopressin-stimulated phospholipid methyltransferase phosphorylation was time- and dose-dependent. 32P-labeled phospholipid methyltransferase was recovered by immunoprecipitation and SDS-polyacrylamide gel electrophoresis. After electrophoresis, phospholipid methyltransferase was electroeluted from the polyacrylamide gel and subjected to tryptic digestion or HCl hydrolysis. Analysis of 32P-labeled peptides reveals only one site of phosphorylation and the analysis of [32P]phosphoamino acids indicates that phosphoserine is the only labeled amino acid.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Feb
|
pubmed:issn |
0014-5793
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:day |
17
|
pubmed:volume |
196
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
274-8
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:3949001-Animals,
pubmed-meshheading:3949001-Enzyme Activation,
pubmed-meshheading:3949001-Liver,
pubmed-meshheading:3949001-Methyltransferases,
pubmed-meshheading:3949001-Phosphatidyl-N-Methylethanolamine N-Methyltransferase,
pubmed-meshheading:3949001-Phosphatidylethanolamine N-Methyltransferase,
pubmed-meshheading:3949001-Phosphorylation,
pubmed-meshheading:3949001-Phosphoserine,
pubmed-meshheading:3949001-Rats,
pubmed-meshheading:3949001-Rats, Inbred Strains,
pubmed-meshheading:3949001-Vasopressins
|
pubmed:year |
1986
|
pubmed:articleTitle |
Vasopressin-stimulated phosphorylation of rat liver phospholipid methyltransferase in isolated hepatocytes.
|
pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
|