Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1985-3-29
|
| pubmed:abstractText |
The effects of recombinant interferon gamma (IFN-gamma) on HLA antigen expression were examined in various human carcinoma cell lines: carcinomas of the lung (ChaGo, Oat 75, SK-LC-LL), colon (HT-29, WiDr), larynx (HEp-2), cervix (ME-180) and mammary gland (AlAb). Surface expression of HLA antigens was determined using monoclonal antibodies directed against monomorphic determinants of HLA class II (HD II, 2.06) and HLA class I (W6/32) by several techniques including indirect immunofluorescence, immunoperoxidase assay, radioimmunoassay of cells, and immunoprecipitation of radioiodinated cells. Without previous exposure to IFN-gamma, HLA class-II antigen was surface-expressed on a small subpopulation of HEp-2 only. All cell lines examined, except AlAb, could be induced to cell-surface expression of HLA class-II antigen after 48 hr incubation in the presence of IFN-gamma (10 units/ml). HLA class-I antigen was present on all cell lines even without treatment, and it was increased after IFN-exposure. Incorporation of 3H-thymidine and 3H-leucine and expression of the transferrin receptor were not significantly altered by IFN-gamma application, indicating a specific regulatory effect of IFN-gamma on HLA antigen expression. Our data demonstrate that HLA class-II surface expression is inducible by IFN-gamma in a variety of epithelial tumor lines. This may have implications for the host immune system defense mechanisms against tumors.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0020-7136
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
35
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
245-50
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:3918946-Breast Neoplasms,
pubmed-meshheading:3918946-Carcinoma,
pubmed-meshheading:3918946-Cell Line,
pubmed-meshheading:3918946-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:3918946-Female,
pubmed-meshheading:3918946-Fluorescent Antibody Technique,
pubmed-meshheading:3918946-HLA Antigens,
pubmed-meshheading:3918946-Histocompatibility Antigens Class II,
pubmed-meshheading:3918946-Humans,
pubmed-meshheading:3918946-Immunoenzyme Techniques,
pubmed-meshheading:3918946-Interferon-gamma,
pubmed-meshheading:3918946-Laryngeal Neoplasms,
pubmed-meshheading:3918946-Lung Neoplasms,
pubmed-meshheading:3918946-Uterine Cervical Neoplasms
|
| pubmed:year |
1985
|
| pubmed:articleTitle |
Induction of HLA class-II antigen expression on human carcinoma cell lines by IFN-Gamma.
|
| pubmed:publicationType |
Journal Article
|