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rdf:type |
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lifeskim:mentions |
|
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pubmed:issue |
5
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|
pubmed:dateCreated |
1986-3-4
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|
pubmed:abstractText |
A variant of the microtitre GM1-ELISA for Escherichia coli heat-labile enterotoxin was studied. The test was improved by both reducing the assay time from 2 1/2 d to 8 h and by determining the most appropriate GM1 coating concentration. Coating the plates with greater than or equal to 3 micrograms of GM1/ml yielded a maximal sensitivity and ensured a linear relationship between the enterotoxin concentration and the extinction observed when using the final assay-procedure. Thus an optimal accuracy was obtained. This ELISA was 4- to 8-times more sensitive than the Vero cell monolayer assay. The sensitivity of this ELISA and of the chinese hamster ovary cell monolayer assay were identical.
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pubmed:language |
eng
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pubmed:journal |
|
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pubmed:citationSubset |
IM
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pubmed:chemical |
|
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
|
|
pubmed:issn |
0021-8847
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:volume |
59
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
443-9
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pubmed:dateRevised |
2008-8-20
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pubmed:meshHeading |
pubmed-meshheading:3910634-Animals,
pubmed-meshheading:3910634-Bacterial Toxins,
pubmed-meshheading:3910634-Cell Line,
pubmed-meshheading:3910634-Cercopithecus aethiops,
pubmed-meshheading:3910634-Enterotoxins,
pubmed-meshheading:3910634-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:3910634-Escherichia coli,
pubmed-meshheading:3910634-Escherichia coli Proteins,
pubmed-meshheading:3910634-G(M1) Ganglioside,
pubmed-meshheading:3910634-Kidney,
pubmed-meshheading:3910634-Microchemistry
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pubmed:year |
1985
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pubmed:articleTitle |
Improvements in the microtitre GM1 ganglioside enzyme-linked immunosorbent assay for Escherichia coli heat-labile enterotoxin.
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pubmed:publicationType |
Journal Article
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