Linked Life Data

3910131

Source:http://linkedlifedata.com/resource/pubmed/id/3910131

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1986-3-5
pubmed:abstractText
A quantitative method for circulating islet cell surface antibodies (ICSA), based on the binding of 125I-protein A to insulin-producing RINm5F cells, was used to evaluate ICSA in plasma of 4- to 40-week-old Aston obese hyperglycaemic (ob/ob) mice and normal control (+/+) mice. RINm5F cells bound 2502 +/- 1196 c.p.m. 125I-protein A per 10(5) cells (mean +/- S.D., n = 54) after incubation with +/+ plasma. ICSA positive plasma (defined as 125I-protein A binding, mean +/- 2 S.D. of +/+ plasma) was detected in 3 out of 54 +/+ mice and 3 out of 54 ob/ob mice. ICSA were not observed in ob/ob mice before the onset of diabetes (7 weeks of age), but were detected at 9, 20 and 40 weeks. At 20 weeks 125I-protein A binding produced by ob/ob plasma was 35% greater than +/+ plasma (P less than 0.05). The low occurrence of ICSA in ob/ob mice (6%) suggests that factors other than ICSA are responsible for B-cell dysfunction and eventual islet degeneration observed in Aston ob/ob mice.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0144-8463
pubmed:author
pubmed:issnType
Print
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
715-20
pubmed:dateRevised
2003-11-14
pubmed:meshHeading
pubmed:year
1985
pubmed:articleTitle
Islet cell surface antibodies in genetically obese hyperglycaemic (ob/ob) mice.
pubmed:publicationType
Journal Article