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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1979-8-16
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pubmed:abstractText |
Saccharomyces cerevisiae cells contain a small internal pool of the secretory enzymes invertase and acid phosphatase. This pool increases up to 8-fold at 37 degrees C in a temperature-sensitive, secretion-defective mutant strain (sec 1-1). Cell division and incorporation of a sulfate permease activity stop abruptly at the restrictive temperature, while protein synthesis continues for several hours. Electron microscopy of mutant cells incubated at 37 degrees C reveals a large increase in the number of intracellular membrane-bound vesicles, which are shown by histochemical staining to contain the accumulated acid phosphatase. The vesicles are removed and the accumulated enzymes are secreted when cells are returned to a permissive temperature in the presence or absence of cycloheximide. These results are consistent with a vesicle intermediate in the yeast secretory pathway and suggest that exocytosis may contribute to cell-surface growth.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/377286-1096303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/377286-1100612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/377286-1164141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/377286-16561177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/377286-16592617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/377286-199574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/377286-237205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/377286-239923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/377286-323239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/377286-328492,
http://linkedlifedata.com/resource/pubmed/commentcorrection/377286-348562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/377286-4554644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/377286-5276773,
http://linkedlifedata.com/resource/pubmed/commentcorrection/377286-5328640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/377286-5601020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/377286-837255
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
76
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1858-62
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pubmed:dateRevised |
2010-9-2
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pubmed:meshHeading |
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pubmed:year |
1979
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pubmed:articleTitle |
Secretion and cell-surface growth are blocked in a temperature-sensitive mutant of Saccharomyces cerevisiae.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, Non-P.H.S.
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