Linked Life Data

3713526

Source:http://linkedlifedata.com/resource/pubmed/id/3713526

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1986-7-16
pubmed:abstractText
Using established tissue culture methodology, a protocol has been developed for the culture of specific hypothalamic regions. A microdissection technique is used to remove the paraventricular and supraoptic nuclear regions from fresh tissue. These explants are maintained in culture and appear to be viable on the basis of several criteria: the continuing presence of peptides in both tissue and media, the ability to incorporate a labeled amino acid precursor into proteins and peptides, the histological appearance using phase contrast microscopy, and the immunocytochemical identification of peptide neurons. This tissue culture model may prove useful in the study of neuroendocrine mechanisms in specific brain regions.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0076-6879
pubmed:author
pubmed:issnType
Print
pubmed:volume
124
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
359-71
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
A tissue culture model for the study of peptide synthesis and secretion from microdissected hypothalamic explants.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't