| pubmed-article:3519659 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:3519659 | lifeskim:mentions | umls-concept:C0039128 | lld:lifeskim |
| pubmed-article:3519659 | lifeskim:mentions | umls-concept:C0086231 | lld:lifeskim |
| pubmed-article:3519659 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:3519659 | pubmed:dateCreated | 1986-6-27 | lld:pubmed |
| pubmed-article:3519659 | pubmed:abstractText | An enzyme immunoassay for the diagnosis of syphilis (ELISA-SY) was developed with solid-phase extracts of Treponema pallidum, specimen diluent containing Reiter treponeme absorbent, and three 30-min incubations. The ELISA-SY results were determined in comparison with a standardized positive control and reported as a percentage of strong positive control. In tests with 1,005 serum samples from a venereal disease clinic and other sources, 98.2% agreement was found with fluorescent treponemal antibody-absorption (FTA-ABS) results, and 98.3% agreement was found with T. pallidum passive hemagglutination (PHA) findings. Only 1 of 29 sera originally considered to be biologically false-positive by ELISA-SY; the latter specimen was also positive by PHA and FTA-ABS tests performed in our laboratories. Serum samples from clinically diagnosed syphilitics (16 primary-stage isolates, 7 secondary-stage isolates, and 3-latent-stage isolates) were all positive by ELISA-SY, FTA-ABS, and PHA. Serum samples from 51 newborns suspected of having syphilis on the basis of positive cardiolipin flocculation tests showed 98% agreement of ELISA-SY results with FTA-ABS and PHA findings. Sera from all 61 patients with a variety of autoimmune and other diseases known to be associated with biologically false-positive reactions for syphilis were negative by this ELISA-SY. The specificity of the ELISA procedure for T. pallidum antibody was also confirmed immunologically by blocking experiments. | lld:pubmed |
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| pubmed-article:3519659 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3519659 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:3519659 | pubmed:language | eng | lld:pubmed |
| pubmed-article:3519659 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3519659 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:3519659 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3519659 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:3519659 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:3519659 | pubmed:month | May | lld:pubmed |
| pubmed-article:3519659 | pubmed:issn | 0095-1137 | lld:pubmed |
| pubmed-article:3519659 | pubmed:author | pubmed-author:PAZL MLM | lld:pubmed |
| pubmed-article:3519659 | pubmed:author | pubmed-author:HalbertS PSP | lld:pubmed |
| pubmed-article:3519659 | pubmed:author | pubmed-author:ChenJJ | lld:pubmed |
| pubmed-article:3519659 | pubmed:author | pubmed-author:SchubertC MCM | lld:pubmed |
| pubmed-article:3519659 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:3519659 | pubmed:volume | 23 | lld:pubmed |
| pubmed-article:3519659 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:3519659 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:3519659 | pubmed:pagination | 876-80 | lld:pubmed |
| pubmed-article:3519659 | pubmed:dateRevised | 2010-9-9 | lld:pubmed |
| pubmed-article:3519659 | pubmed:meshHeading | pubmed-meshheading:3519659-... | lld:pubmed |
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| pubmed-article:3519659 | pubmed:meshHeading | pubmed-meshheading:3519659-... | lld:pubmed |
| pubmed-article:3519659 | pubmed:year | 1986 | lld:pubmed |
| pubmed-article:3519659 | pubmed:articleTitle | Treponemal antibody-absorbent enzyme immunoassay for syphilis. | lld:pubmed |
| pubmed-article:3519659 | pubmed:publicationType | Journal Article | lld:pubmed |
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