Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1986-6-27
|
| pubmed:abstractText |
An enzyme immunoassay for the diagnosis of syphilis (ELISA-SY) was developed with solid-phase extracts of Treponema pallidum, specimen diluent containing Reiter treponeme absorbent, and three 30-min incubations. The ELISA-SY results were determined in comparison with a standardized positive control and reported as a percentage of strong positive control. In tests with 1,005 serum samples from a venereal disease clinic and other sources, 98.2% agreement was found with fluorescent treponemal antibody-absorption (FTA-ABS) results, and 98.3% agreement was found with T. pallidum passive hemagglutination (PHA) findings. Only 1 of 29 sera originally considered to be biologically false-positive by ELISA-SY; the latter specimen was also positive by PHA and FTA-ABS tests performed in our laboratories. Serum samples from clinically diagnosed syphilitics (16 primary-stage isolates, 7 secondary-stage isolates, and 3-latent-stage isolates) were all positive by ELISA-SY, FTA-ABS, and PHA. Serum samples from 51 newborns suspected of having syphilis on the basis of positive cardiolipin flocculation tests showed 98% agreement of ELISA-SY results with FTA-ABS and PHA findings. Sera from all 61 patients with a variety of autoimmune and other diseases known to be associated with biologically false-positive reactions for syphilis were negative by this ELISA-SY. The specificity of the ELISA procedure for T. pallidum antibody was also confirmed immunologically by blocking experiments.
|
| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3519659-1085667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3519659-1098730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3519659-4568465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3519659-4983444,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3519659-5135623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3519659-5335572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3519659-561820,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3519659-6055766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3519659-6370510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3519659-6394613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3519659-6752188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3519659-7034857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3519659-7040460
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0095-1137
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
23
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
876-80
|
| pubmed:dateRevised |
2010-9-9
|
| pubmed:meshHeading |
pubmed-meshheading:3519659-Antibodies,
pubmed-meshheading:3519659-Antibodies, Bacterial,
pubmed-meshheading:3519659-Antibody Specificity,
pubmed-meshheading:3519659-Autoimmune Diseases,
pubmed-meshheading:3519659-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:3519659-Humans,
pubmed-meshheading:3519659-Serologic Tests,
pubmed-meshheading:3519659-Syphilis,
pubmed-meshheading:3519659-Treponema pallidum
|
| pubmed:year |
1986
|
| pubmed:articleTitle |
Treponemal antibody-absorbent enzyme immunoassay for syphilis.
|
| pubmed:publicationType |
Journal Article
|