Linked Life Data

3467783

Source:http://linkedlifedata.com/resource/pubmed/id/3467783

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1987-2-24
pubmed:abstractText
Growth factor activity which stimulates anchorage-independent growth (AIG) in a rat fibroblast line, was detected in human premalignant adenoma tissue from familial polyposis coli colectomy specimens and in serum-free culture supernatant from an adenoma cell line PC/AA. The activity extracted from adenoma tissue was compared quantitatively in the AIG bioassay with extracts of normal mucosa from split thickness colorectal tissue. Adenoma tissue yielded three times the amount of acid-extractable protein g-1 wet wt and adenoma extracts consistently had significantly greater specific activity over a wide protein concentration range. Activity extracted from adenoma tissue and from the derived cell line PC/AA were compared qualitatively after fractionation by gel filtration. Both extracts showed almost identical profiles of biological activity after assay of individual fractions for AIG stimulation, suggesting that the factor(s) originates from the epithelial component of the adenoma tissue since PC/AA is a pure epithelial cell line. Activity eluted as two major peaks with apparent mol. wts of 9 kd and 20-25 kd (relative to standards) in both cases. This report demonstrates for the first time that elevated production of a growth factor may be an early change in the evolution of human colorectal cancer from small, premalignant adenomas.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/3467783-3002607, http://linkedlifedata.com/resource/pubmed/commentcorrection/3467783-3002610, http://linkedlifedata.com/resource/pubmed/commentcorrection/3467783-3900102, http://linkedlifedata.com/resource/pubmed/commentcorrection/3467783-3904801, http://linkedlifedata.com/resource/pubmed/commentcorrection/3467783-3904802, http://linkedlifedata.com/resource/pubmed/commentcorrection/3467783-6130835, http://linkedlifedata.com/resource/pubmed/commentcorrection/3467783-6251462, http://linkedlifedata.com/resource/pubmed/commentcorrection/3467783-6299535, http://linkedlifedata.com/resource/pubmed/commentcorrection/3467783-6482968, http://linkedlifedata.com/resource/pubmed/commentcorrection/3467783-6746117, http://linkedlifedata.com/resource/pubmed/commentcorrection/3467783-7103997
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0007-0920
pubmed:author
pubmed:issnType
Print
pubmed:volume
54
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
799-805
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
Elevated production of growth factor by human premalignant colon adenomas and a derived epithelial cell line.
pubmed:publicationType
Journal Article, Comparative Study