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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1988-10-27
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pubmed:abstractText |
Prior studies by our laboratory, utilizing the 1,2-dimethylhydrazine experimental model of colonic cancer, had shown that administration of this procarcinogen for 5 weeks was found to increase phospholipid methyltransferase activity and the fluidity of rat distal colonic brush-border membranes. The present studies were conducted to further explore these 'premalignant' colonic phenomena. Male albino rats of the Sherman strain were subcutaneously injected with dimethylhydrazine (20 mg/kg body weight per week) or diluent for 5 weeks. Animals from each group were killed, distal colonic tissue harvested and the levels of S-adenosylmethionine, S-adenosylhomocysteine and decarboxylated S-adenosylmethionine measured by high performance liquid chromatography. The activity of methionine adenosyltransferase was also examined in these tissues. Additionally, brush-border membranes were isolated from the distal colonocytes of control and treated-animals and examined and compared with respect to their phospholipid methylation activities as well as their lipid fluidity as assessed by the rotational mobilities of the probes 1,6-diphenyl-1,3,5-hexatriene and DL-12-(9-anthroyl)stearic acid and translational mobility of the fluorophore pyrenedecanoic acid. The results of these studies demonstrated: (1) phospholipid methyltransferase activity in rat colonic plasma membranes was increased concomitantly with increases in the cellular levels of S-adenosylmethionine and the S-adenosylmethionine/S-adenosylhomocysteine ratio in the distal colonic segment of treated-animals; and (2) the lateral diffusion of rat distal colonic brush-border membrane lipids, as assessed by the ratio of excimer/monomer fluorescence intensities of the fluorophore pyrenedecanoate, was also increased after dimethylhydrazine administration to these animals for 5 weeks.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1,2-Dimethylhydrazine,
http://linkedlifedata.com/resource/pubmed/chemical/Decanoic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Dimethylhydrazines,
http://linkedlifedata.com/resource/pubmed/chemical/Homocysteine,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Lipids,
http://linkedlifedata.com/resource/pubmed/chemical/Methionine Adenosyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/Methylhydrazines,
http://linkedlifedata.com/resource/pubmed/chemical/S-Adenosylhomocysteine,
http://linkedlifedata.com/resource/pubmed/chemical/S-Adenosylmethionine,
http://linkedlifedata.com/resource/pubmed/chemical/pyrenedecanoic acid
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0006-3002
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
944
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
101-7
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:3415996-1,2-Dimethylhydrazine,
pubmed-meshheading:3415996-Animals,
pubmed-meshheading:3415996-Colonic Neoplasms,
pubmed-meshheading:3415996-Decanoic Acids,
pubmed-meshheading:3415996-Diffusion,
pubmed-meshheading:3415996-Dimethylhydrazines,
pubmed-meshheading:3415996-Homocysteine,
pubmed-meshheading:3415996-Membrane Lipids,
pubmed-meshheading:3415996-Methionine Adenosyltransferase,
pubmed-meshheading:3415996-Methylhydrazines,
pubmed-meshheading:3415996-Precancerous Conditions,
pubmed-meshheading:3415996-Rats,
pubmed-meshheading:3415996-S-Adenosylhomocysteine,
pubmed-meshheading:3415996-S-Adenosylmethionine
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pubmed:year |
1988
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pubmed:articleTitle |
1,2-Dimethylhydrazine-induced premalignant alterations in the S-adenosylmethionine/S-adenosylhomocysteine ratio and membrane lipid lateral diffusion of the rat distal colon.
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pubmed:affiliation |
Department of Medicine, University of Chicago, IL.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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