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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1988-8-29
|
| pubmed:abstractText |
A high pressure liquid chromatographic (HPLC) method with internal analogue standardization for the determination of 5-hydroxypropafenone in plasma is described. The method comprises extraction from plasma at pH 9 with diethyl-ether and quantification after HPLC separation using a reverse phase by means of electrochemical (reductive) detection after electrochemical preoxidation. When using 1 ml plasma the lower limit of detection is 0.5 ng/ml. Under routine conditions the limit of determination is estimated to be lower than 2 ng/ml. The variation coefficients of duplicates drop from about 10% in the range of the determination limit to about 3% at 5 ng/ml and above. The determination of 5-hydroxypropafenone is not impaired by other known metabolites of propafenone.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0379-0355
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
10
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
319-29
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading | |
| pubmed:year |
1988
|
| pubmed:articleTitle |
HPLC determination of 5-hydroxypropafenone in plasma with electrochemical detection.
|
| pubmed:affiliation |
Knoll AG, Dept. Biochemistry, Ludwigshafen/Rhein, FRG.
|
| pubmed:publicationType |
Journal Article
|