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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1988-8-26
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pubmed:databankReference | |
pubmed:abstractText |
A cDNA library from the mink pituitary was screened using as probe a synthetic oligodeoxyribonucleotide, 5'-TTCATGACCTCCGA-3', corresponding to the endorphin region of bovine proopiomelanocortin (POMC) cDNA. As a result, several clones containing inserts complementary to POMC mRNA were identified. The sequence of one of the fragments (585 bp, 65% of the total length of mRNA) was determined. A high degree of homology (over 80%) among the primary structures of sequences from mink, man, and bovine cDNA POMC was established. With the cloned mink cDNA fragment as probe, the DNAs from mink-Chinese hamster hybrid clones were studied. The results of segregation analysis of mink POMC sequences and mink chromosomes in the mink-Chinese hamster panel allowed us to assign the POMC gene to mink chromosome 11.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0888-7543
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
185-8
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:3397057-Amino Acid Sequence,
pubmed-meshheading:3397057-Animals,
pubmed-meshheading:3397057-Cattle,
pubmed-meshheading:3397057-Chromosome Mapping,
pubmed-meshheading:3397057-Cloning, Molecular,
pubmed-meshheading:3397057-Cricetinae,
pubmed-meshheading:3397057-Cricetulus,
pubmed-meshheading:3397057-DNA,
pubmed-meshheading:3397057-Humans,
pubmed-meshheading:3397057-Hybrid Cells,
pubmed-meshheading:3397057-Mink,
pubmed-meshheading:3397057-Molecular Sequence Data,
pubmed-meshheading:3397057-Nucleic Acid Hybridization,
pubmed-meshheading:3397057-Pro-Opiomelanocortin,
pubmed-meshheading:3397057-Sequence Homology, Nucleic Acid
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pubmed:year |
1988
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pubmed:articleTitle |
The mink proopiomelanocortin gene: characterization of cDNA and chromosomal localization.
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pubmed:affiliation |
Institute of Cytology and Genetics, Academy of Sciences of the USSR, Siberian Department, Novosibirsk.
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pubmed:publicationType |
Journal Article
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