Linked Life Data

3337246

Source:http://linkedlifedata.com/resource/pubmed/id/3337246

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1 Pt 2
pubmed:dateCreated
1988-2-25
pubmed:abstractText
Platelet-activating factor (PAF) and platelet-derived growth factor (PDGF) likely play important roles in glomerular processes. To identify mechanisms of PAF- and PDGF-induced mesangial cell activation, we characterized effects of both agents on cytosolic free [Ca2+] ([Ca2+]f) and phospholipase activation. When cultured rat renal mesangial cells were stimulated by PAF, [Ca2+]f increased (within 10 s) from 110 +/- 6 to 209 +/- 14 nM, due to release of Ca2+ from intracellular storage sites, as well as entry of Ca2+ from external milieu. PAF also rapidly increased free arachidonate, diacylglycerol, and inositol trisphosphate (IP3) levels. PDGF increased [Ca2+]f from 78 +/- 12 to 192 +/- 22 nM, but the time response was different from that seen with PAF. Peak [Ca2+]f increase occurred at approximately 1 min subsequent to stimulation. Like PAF, increased [Ca2+]f was due to release of Ca2+ from intracellular stores and entry from extracellular media. PDGF increased levels of free arachidonate prior to an increase in diacylglycerol levels. PDGF increased inositol bisphosphate and IP3 levels at 2 min but not at 15 s. Thus, PDGF may mediate a phospholipase C-independent activation of phospholipase A2. In permeabilized mesangial cells, IP3 released Ca2+ from nonmitochondrial storage sites. Thus, PAF- and PDGF-induced increases in [Ca2+]f were likely secondary to activation of phospholipase C, resulting in increased levels of IP3. Understanding differences in mechanisms of mesangial cell activation by PAF and PDGF will likely lead to a better understanding of signal transduction pathways and provide insight into the role of these activators in glomerular disease.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
254
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
F87-94
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
PAF and PDGF increase cytosolic [Ca2+] and phospholipase activity in mesangial cells.
pubmed:affiliation
Medical Services, Massachusetts General Hospital, Boston.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't