Linked Life Data

3262792

Source:http://linkedlifedata.com/resource/pubmed/id/3262792

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1988-11-17
pubmed:abstractText
Changes in the concentration of cytosolic free calcium ((Ca2+)i) in response to purified blood monocyte IL-1 and human rIL-1 alpha and rIL-1 beta (17.5 kDa) were measured in murine L-M fibroblasts and in human foreskin fibroblasts using the fluorescent Ca2+ indicator, fura-2. In L-M fibroblasts, each of these IL-1 species, but not a recombinant 24-kDa precursor of the predominant IL-1 beta, produced a prompt, dose-related, and transient increase in (Ca2+)i. The effect was smaller but not eliminated when the cells were stimulated in EGTA-containing calcium, suggesting that the rise in (Ca2+)i was due to influx from both intracellular and extracellular Ca2+ pools. In human fibroblasts, however, the (Ca2+)i increased gradually, reaching a maximum after 1 hr of incubation with IL-1 and returning slowly to near basal levels in the following 2 hr. In contrast to the L-M cells, this accumulation of Ca2+ was abolished by EGTA, suggesting that in human fibroblasts, Ca2+ is mobilized solely from the extracellular space. Addition of the Ca2+ channel blockers verapamil and nifedipine was ineffective. IL-1 alpha and IL-1 beta both induced a dose-related increase in prostaglandin E2, but only in the human fibroblasts. These findings indicate that an increase in (Ca2+)i may be an important second mediator by which IL-1 initiates cell activation, but the signal may differ between cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0887-6924
pubmed:author
pubmed:issnType
Print
pubmed:volume
2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
691-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Effects of natural and recombinant interleukin-1 alpha and -beta on cytosolic free calcium in human and murine fibroblasts.
pubmed:affiliation
Department of Clinical Chemistry and Medicine C, Herlev Hospital, University of Copenhagen, Denmark.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't