Linked Life Data

3233210

Source:http://linkedlifedata.com/resource/pubmed/id/3233210

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
25
pubmed:dateCreated
1989-5-1
pubmed:abstractText
We have measured the base-pair lifetimes in oligodeoxynucleotides containing tracts of A.T base pairs using imino proton magnetic resonance. When the tract contains more than four consecutive A.T base pairs, possibly including a 5'-AT step but not a 5'-TA step, anomalously long lifetimes are observed. For example, the lifetimes of the central A.T base pairs of the dodecamer 5'-d-CGCAAAAAAGCG are 122 and 91 ms at 15 degrees C whereas, in the same conditions, the lifetime of the central A.T pair of the decamer 5'-d-CGCGATCGCG is only 4 ms, a value similar to those measured in several other B-DNA oligoduplexes [Leroy et al. (1988) J. Mol. Biol. 200, 223-238]. This strongly suggests that, in tracts of four A.T pairs or more, a conformation distinct from standard B-DNA is formed cooperatively. All sequences known to generate curved DNA exhibit anomalously long base-pair lifetimes. This is the first local and physical property shown to correlate with DNA curvature. Our observations suggest that the structure responsible for the long lifetimes is involved in the curvature of DNA.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
13
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8894-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Evidence from base-pair kinetics for two types of adenine tract structures in solution: their relation to DNA curvature.
pubmed:affiliation
Groupe de Biophysique, Ecole Polytechnique, Palaiseau, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't