Linked Life Data

3198645

Source:http://linkedlifedata.com/resource/pubmed/id/3198645

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
36
pubmed:dateCreated
1989-1-24
pubmed:abstractText
Protein candidates for the attachment of DNA within eukaryotic cell nuclei were identified by isolating nuclear matrix proteins and determining which of those proteins co-sedimented with DNA within a 5.7 M CsCl gradient. The presence of attached nucleic acid was detected after the proteins were subjected to the denaturing conditions of isoelectric focusing/sodium dodecyl sulfate two-dimensional polyacrylamide gel electrophoresis. The attached nucleic acid was detected with silver staining, ethidium bromide, and Amido Black binding. The nucleic acid was identified as DNA based on its ability to be labeled in vitro by terminal deoxynucleotidyltransferase and DNA polymerase I (Klenow). Three proteins were identified as containing attached DNA, one of which was vimentin. The proteins had apparent Mr and pI values of 70,000, 4.3; 70,000, 5.3; and 57,000, 4.8, respectively. We propose that these proteins are within a class of nuclear proteins containing firmly attached DNA and have referred to them as DNA attachment proteins.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
263
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
19678-83
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Identification of attachment proteins for DNA in Chinese hamster ovary cells.
pubmed:affiliation
Department of Radiation Oncology, University of Arizona Cancer Center, Tucson 85724.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.