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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1-2
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pubmed:dateCreated |
1988-9-16
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pubmed:abstractText |
The effects of normal sera from humans, rats, and guinea pigs on unsensitized rat peritoneal mast cells were studied in vitro. Five to 20% fresh human sera induced mast cell death and substantial histamine release. The factor was heat labile. Neither hereditary C3-deficient sera nor experimentally C1q-depleted sera showed cytotoxicity. The CH50 activity of human serum was decreased to about one half after a 15-min incubation with 2 X 10(6) mast cells/ml at 37 degrees C. The cytotoxic activity and CH50 reduction were completely eliminated by an addition of 10 mM Mg-EGTA to the serum. These data demonstrated that unsensitized rat mast cells served as both the initiator and target of complement activity when human serum was used as a complement source. Requirements of both Ca++ and C1q suggested the activation of the classical pathway of complement. Fresh 5-20% sera from rats and guinea pigs, on the other hand, showed neither cytotoxicity nor CH50 reduction. Furthermore, these sera strongly inhibited the human serum-induced reaction. The latter results indicated the presence of a modulating factor in rat and guinea pig sera, which inhibits mast cell associated complement activation.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0065-4299
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
73-9
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:3136626-Animals,
pubmed-meshheading:3136626-Blood Physiological Phenomena,
pubmed-meshheading:3136626-Calcium,
pubmed-meshheading:3136626-Complement Activation,
pubmed-meshheading:3136626-Complement System Proteins,
pubmed-meshheading:3136626-Egtazic Acid,
pubmed-meshheading:3136626-Guinea Pigs,
pubmed-meshheading:3136626-Hemolysis,
pubmed-meshheading:3136626-Humans,
pubmed-meshheading:3136626-Male,
pubmed-meshheading:3136626-Mast Cells,
pubmed-meshheading:3136626-Rats
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pubmed:year |
1988
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pubmed:articleTitle |
Activation of human complement by rat peritoneal mast cells and its inhibition by a rat serum factor.
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pubmed:affiliation |
Department of Pathology, Shiga University of Medical Science, Ohtsu, Japan.
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pubmed:publicationType |
Journal Article
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