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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1987-5-15
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pubmed:abstractText |
The site-specific recombinase encoded by the yeast plasmid 2-microns circle (FLP) forms a transient covalent linkage with its substrate DNA via a tyrosine residue, which appears to be located near its COOH terminus. The homology of the COOH terminus of FLP with that of the Int family of recombinases suggests that tyrosine-343 of FLP could be involved in forming the DNA-protein bridge. We have mutated tyrosine-343 to a phenylalanine or serine. We demonstrate that the binding of each of the two mutant proteins to its substrate is indistinguishable from that of wild-type FLP. However, both mutant proteins are incapable of catalyzing strand cleavage and recombination.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3104911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3104911-212715,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3104911-2427927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3104911-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3104911-2995370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3104911-3003022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3104911-3011407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3104911-3016704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3104911-3026152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3104911-3528147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3104911-3537720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3104911-3540590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3104911-3711092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3104911-3796604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3104911-3856690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3104911-3879971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3104911-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3104911-3898075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3104911-6099239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3104911-6220808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3104911-6246368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3104911-6250048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3104911-6251374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3104911-6251543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3104911-6269755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3104911-6269756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3104911-6301692,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3104911-6306561,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3104911-6308572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3104911-6315399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3104911-6326835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3104911-6457725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3104911-6597765
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0027-8424
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:volume |
84
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2189-93
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1987
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pubmed:articleTitle |
Mutations in the 2-microns circle site-specific recombinase that abolish recombination without affecting substrate recognition.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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