Linked Life Data

3086351

Source:http://linkedlifedata.com/resource/pubmed/id/3086351

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1986-7-17
pubmed:abstractText
The authors propose the use of specific sensors immobilized by ligands onto artificial supports, and the elaboration of a computerized system for the determination of various antigens, haptens or antibodies in biological fluids according to enzyme-linked immunosorbent assay techniques. Two enzymes are applied in this technique: the first (ribonuclease) for reversibly linking the immunocomplex to the insoluble support via disulphur bridges; the second (beta-D-glucose oxidase) for labelling the antigen. Enzyme activity is measured in the presence of glucose oxidase by fixing the immunocomplex onto a pO2 electrode. After incubation of the antigen labelled with glucose oxidase and the free antigen with specific antibodies linked with ribonuclease, to reduce the pre-established concentration, the reaction medium is introduced into the continuous flow cell. O2 consumption due to the enzyme reaction is measured by the actual time that the electrode is in contact with a glucose standard solution. Cleavage of the disulphur bridges is caused by an injection of dithiothreitol solution. Treatment of the signal obtained is realized with an automatic microcomputer system. The preliminary results show that reproducibility with the same membrane for ten measurements is less than 5%. Elution performed using dithiothreitol for example, shows that cleavage between the immunocomplex and the thiol-containing support is obtained after a few minutes, and 98% of the immunocomplex is eluted.
pubmed:language
fre
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, http://linkedlifedata.com/resource/pubmed/chemical/Benzoquinones, http://linkedlifedata.com/resource/pubmed/chemical/Carbodiimides, http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/Gelatin, http://linkedlifedata.com/resource/pubmed/chemical/Glucose, http://linkedlifedata.com/resource/pubmed/chemical/Glucose Oxidase, http://linkedlifedata.com/resource/pubmed/chemical/Glutaral, http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G, http://linkedlifedata.com/resource/pubmed/chemical/Membranes, Artificial, http://linkedlifedata.com/resource/pubmed/chemical/Quinones, http://linkedlifedata.com/resource/pubmed/chemical/Ribonucleases, http://linkedlifedata.com/resource/pubmed/chemical/benzoquinone
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9673
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
376
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
199-210
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1986
pubmed:articleTitle
[Immobilization of enzymatic inhibitors for the isolation of reversible immunologic sensors].
pubmed:publicationType
Journal Article, English Abstract