Linked Life Data

3046617

Source:http://linkedlifedata.com/resource/pubmed/id/3046617

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1988-10-5
pubmed:abstractText
A mutant (erg6) of Saccharomyces cerevisiae defective in S-adenosylmethionine: delta 24-sterol-C-methyl transferase (EC2.1.1.41) which normally produces cholesta-5,7,24-trienol and cholesta-5,7,22,24-tetraenol as the major sterols (total 4,4-desmethyl sterol content-8.3 fg/cell) was shown to synthesize trace levels of cholesterol (0.08 fg/cell). The identity of cholesterol was established by co-chromatography in TLC, GLC and HPLC with an authentic sample, mass spectroscopy and after an incubation with [1-14C]acetate by isotopic dilution and recrystallization of the radiochemically purified material to constant specific activity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
30
pubmed:volume
155
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
509-17
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1988
pubmed:articleTitle
Biosynthesis of cholesterol in the yeast mutant erg6.
pubmed:affiliation
Russell Research Center, U.S. Department of Agriculture, Athens, GA 30613.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't