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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1986-12-17
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pubmed:abstractText |
The purE operon of Escherichia coli has been cloned and localized to a 1.7-kb HpaI fragment. The operon has been further characterized by subcloning into the lac fusion vector, pMC1403, and by the construction of BAL 31-generated deletions. The purE regulation region has been identified by assay of beta-galactosidase produced by pur-lac fusion plasmids and by RNA polymerase binding to end-labelled restriction fragments. Two purE promoters have been identified; one strong that is regulated by purines, the other weaker which is not regulated. The latter may be internal to the purE1 structural gene.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0378-1119
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
44
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
55-62
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:3021590-Cloning, Molecular,
pubmed-meshheading:3021590-DNA Restriction Enzymes,
pubmed-meshheading:3021590-DNA-Directed RNA Polymerases,
pubmed-meshheading:3021590-Escherichia coli,
pubmed-meshheading:3021590-Genes,
pubmed-meshheading:3021590-Genes, Bacterial,
pubmed-meshheading:3021590-Genetic Complementation Test,
pubmed-meshheading:3021590-Genetic Vectors,
pubmed-meshheading:3021590-Operon,
pubmed-meshheading:3021590-Plasmids,
pubmed-meshheading:3021590-Promoter Regions, Genetic,
pubmed-meshheading:3021590-beta-Galactosidase
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pubmed:year |
1986
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pubmed:articleTitle |
Molecular cloning and characterization of the purE operon of Escherichia coli.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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