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rdf:type |
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lifeskim:mentions |
umls-concept:C0007635,
umls-concept:C0017262,
umls-concept:C0021467,
umls-concept:C0021469,
umls-concept:C0028953,
umls-concept:C0035736,
umls-concept:C0042776,
umls-concept:C0086418,
umls-concept:C0185117,
umls-concept:C0205228,
umls-concept:C0332307,
umls-concept:C0598312,
umls-concept:C1883254,
umls-concept:C2911684
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pubmed:issue |
12
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pubmed:dateCreated |
1986-7-18
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pubmed:abstractText |
The possibility of using oligodeoxynucleotides complementary to viral RNA or proviral DNA to inhibit the replication of human T-cell lymphotropic virus type III (HTLV-III) [the etiological agent of acquired immunodeficiency syndrome (AIDS)] in cultured human cells was addressed by studying the association of 32P-labeled oligodeoxynucleotides with mammalian cellular components. The results indicated that exogenous oligodeoxynucleotides at 20 microM became associated with the membrane/cytosol fractions of the cell in amounts approximating 1.5 microM. Oligodeoxynucleotides complementary to a region close to the tRNALys primer binding site on HTLV-III RNA and others complementary to HTLV-III mRNA donor or acceptor splice sites inhibited viral replication (assayed as reverse transcriptase) and gene expression (assayed as virus-encoded proteins p15 and p24) by as much as 95%. Use of control (random) oligodeoxynucleotides suggests that the antiviral effects were specific. Although these results pertain to HTLV-III-infected cells in tissue culture, rather than to AIDS patients, they nevertheless point to a therapeutic potential of the complementary oligodeoxynucleotide ("hybridization competition" or "hybridon") approach in the treatment of patients with AIDS and AIDS-related complex.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/3012555-10793670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3012555-200268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3012555-2413364,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3012555-2414659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3012555-2415134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3012555-2578227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3012555-2578615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3012555-2981635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3012555-2982104,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3012555-2990040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3012555-2991599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3012555-2991896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3012555-2994217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3012555-2997922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3012555-3003749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3012555-3006077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3012555-3010316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3012555-4327004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3012555-6095086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3012555-6095449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3012555-6200936,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3012555-6207433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3012555-6246368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3012555-6323013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3012555-6438633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3012555-6438637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3012555-7374461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3012555-75545,
http://linkedlifedata.com/resource/pubmed/commentcorrection/3012555-75546
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
83
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4143-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1986
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pubmed:articleTitle |
Inhibition of replication and expression of human T-cell lymphotropic virus type III in cultured cells by exogenous synthetic oligonucleotides complementary to viral RNA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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