| pubmed-article:2989287 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2989287 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:2989287 | lifeskim:mentions | umls-concept:C0007452 | lld:lifeskim |
| pubmed-article:2989287 | lifeskim:mentions | umls-concept:C0021853 | lld:lifeskim |
| pubmed-article:2989287 | lifeskim:mentions | umls-concept:C0030956 | lld:lifeskim |
| pubmed-article:2989287 | lifeskim:mentions | umls-concept:C0031640 | lld:lifeskim |
| pubmed-article:2989287 | lifeskim:mentions | umls-concept:C0040005 | lld:lifeskim |
| pubmed-article:2989287 | lifeskim:mentions | umls-concept:C0002518 | lld:lifeskim |
| pubmed-article:2989287 | lifeskim:mentions | umls-concept:C0205681 | lld:lifeskim |
| pubmed-article:2989287 | lifeskim:mentions | umls-concept:C1709915 | lld:lifeskim |
| pubmed-article:2989287 | pubmed:issue | 14 | lld:pubmed |
| pubmed-article:2989287 | pubmed:dateCreated | 1985-8-16 | lld:pubmed |
| pubmed-article:2989287 | pubmed:abstractText | We report here the identification of the amino acid residue which forms the covalent intermediate in the catalytic mechanism of bovine intestinal 5'-nucleotide phosphodiesterase and the sequence of the neighboring amino acids. The active site of 5'-nucleotide phosphodiesterase was labeled using thymidine 5'-[alpha-32P]triphosphate as substrate. A single labeled cyanogen bromide peptide was isolated using reversed-phase high performance liquid chromatography. After subdigestion with endoproteinase Lys-C and chymotrypsin, the entire amino acid sequence of the 60-residue active site peptide was obtained using automated Edman degradation. All of the radioactivity of the active site peptide was localized to a hexapeptide with sequence Thr-Phe-Pro-Asn-His-Tyr. Phosphoamino acid analysis of this peptide indicated that the labeled residue was threonine. We are not aware of any other enzymes in which threonine is phosphorylated as a covalent intermediate in the catalytic mechanism. | lld:pubmed |
| pubmed-article:2989287 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2989287 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:2989287 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:2989287 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2989287 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:2989287 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:2989287 | pubmed:author | pubmed-author:ButlerL GLG | lld:pubmed |
| pubmed-article:2989287 | pubmed:author | pubmed-author:HermodsonMM | lld:pubmed |
| pubmed-article:2989287 | pubmed:author | pubmed-author:ColeD JDJ | lld:pubmed |
| pubmed-article:2989287 | pubmed:author | pubmed-author:BlyttH JHJ | lld:pubmed |
| pubmed-article:2989287 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2989287 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:2989287 | pubmed:volume | 260 | lld:pubmed |
| pubmed-article:2989287 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2989287 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2989287 | pubmed:pagination | 8320-4 | lld:pubmed |
| pubmed-article:2989287 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:2989287 | pubmed:year | 1985 | lld:pubmed |
| pubmed-article:2989287 | pubmed:articleTitle | Amino acid sequence of the active site peptide of bovine intestinal 5'-nucleotide phosphodiesterase and identification of the active site residue as threonine. | lld:pubmed |
| pubmed-article:2989287 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2989287 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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