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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1988-9-20
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pubmed:abstractText |
The influence of tumour promoters and growth factors on glycolysis and on fructose-2,6-bisphosphate concentration was studied in isolated mouse spleen lymphocytes and in purified B-cells. The intracellular concentration of fructose 2,6-bisphosphate and the rate of lactate release were increased 2-3-fold in spleen lymphocytes exposed to active phorbol esters, mitogenic lectins, interleukin 4 or lipopolysaccharide. The maximal effect was observed after 1 h of exposure. In these cells hexose 6-phosphates increased 2-fold and 6-phosphofructo-2-kinase activity remained unchanged after treatment with phorbol 12,13-dibutyrate or with lectins. Exposure of B-cells to phorbol 12,13-dibutyrate, interleukin 4 or lipopolysaccharide increased the glycolytic flux and the concentration of fructose 2,6-bisphosphate without relation to their mitogenic activity. Lymphocytes and rat liver 6-phosphofructo-2-kinase were partially purified using the same procedure. The lymphocyte enzyme was not inhibited by sn-glycerol 3-phosphate in contrast to the potent inhibition observed in liver. Treatment of both enzymes with the catalytic subunit of the cyclic-AMP-dependent protein kinase failed to inactivate 6-phosphofructo-2-kinase from lymphocytes. These differences suggest that lymphocytes and liver contain different forms of this enzyme.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Concanavalin A,
http://linkedlifedata.com/resource/pubmed/chemical/Fructosediphosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Hexosediphosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Lactates,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogens,
http://linkedlifedata.com/resource/pubmed/chemical/Phorbol 12,13-Dibutyrate,
http://linkedlifedata.com/resource/pubmed/chemical/Phorbol Esters,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphofructokinase-1,
http://linkedlifedata.com/resource/pubmed/chemical/fructose 2,6-diphosphate
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0014-2956
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
175
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
317-23
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pubmed:dateRevised |
2007-7-23
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pubmed:meshHeading |
pubmed-meshheading:2969804-Animals,
pubmed-meshheading:2969804-B-Lymphocytes,
pubmed-meshheading:2969804-Concanavalin A,
pubmed-meshheading:2969804-DNA Replication,
pubmed-meshheading:2969804-Fructosediphosphates,
pubmed-meshheading:2969804-Hexosediphosphates,
pubmed-meshheading:2969804-Kinetics,
pubmed-meshheading:2969804-Lactates,
pubmed-meshheading:2969804-Lipopolysaccharides,
pubmed-meshheading:2969804-Liver,
pubmed-meshheading:2969804-Lymphocytes,
pubmed-meshheading:2969804-Mice,
pubmed-meshheading:2969804-Mice, Inbred BALB C,
pubmed-meshheading:2969804-Mitogens,
pubmed-meshheading:2969804-Organ Specificity,
pubmed-meshheading:2969804-Phorbol 12,13-Dibutyrate,
pubmed-meshheading:2969804-Phorbol Esters,
pubmed-meshheading:2969804-Phosphofructokinase-1,
pubmed-meshheading:2969804-Rats,
pubmed-meshheading:2969804-Spleen
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pubmed:year |
1988
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pubmed:articleTitle |
Phorbol 12,13-dibutyrate and mitogens increase fructose 2,6-bisphosphate in lymphocytes. Comparison of lymphocyte and rat-liver 6-phosphofructo-2-kinase.
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pubmed:affiliation |
Instituto de Bioquímica, Facultad de Farmacia, Madrid, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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