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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1988-1-5
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pubmed:abstractText |
Since administration of 6-aminonicotinamide (10 micrograms) to day-4 chick embryos in ovo was shown to induce micromelial limbs, biosynthesis of cartilage-characteristic proteoglycan-H (PG-H) as an important index of limb chondrogenesis was examined in day-7 normal and micromelial hind limbs by biochemical and immunological methods. (1) Metabolic labelling of the micromelial limbs with [6-3H]glucosamine and either [35S]sulphate or [35S]methionine, followed by analyses of labelled PG-H by glycerol density-gradient centrifugation under dissociative conditions, showed a marked reduction in the PG-H synthesis. (2) PG-H synthesized by the micromelial limbs was much lower than that synthesized by the normal limbs in the biosynthetic ratio of chondroitin sulphate to keratan sulphate and glycoprotein-type oligosaccharide, although no significant difference was observed in the immunological properties of these proteoglycans. (3) The degree of sulphation of chondroitin sulphates of PG-H was lowered in the micromelial limbs as judged by the increase of unsulphated disaccharide (delta Di-OS) released by chrondroitinase ABC digestion, although there were no significant differences between the normal and the micromelial limbs in the average molecular size (Mr = 38,000) of labelled chondroitin sulphates of PG-H. (4) Addition of beta-D-xyloside, an artificial initiator for chondroitin sulphate synthesis, to the micromelial limbs in culture recovered the incorporation of labelled glucosamine into chondroitin sulphate to that comparable with the normal control with beta-D-xyloside, although the incorporation of [35S]sulphate was lower in the micromelia than in the control with beta-D-xyloside. These results suggest that the reduction in the biosynthesis of the PG-H as well as the production of altered forms of PG-H induced by 6-aminonicotinamide during a critical period of limb morphogenesis may be an important factor for the micromelia.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-1269836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-128147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-13419444,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-13525598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-135762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-13587524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-13662314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-13971270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-14328419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-230515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-234443,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-3096984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-3988733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-4052010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-4226284,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-4231029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-4256054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-4258509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-4261182,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-4261845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-4261846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-4277590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-499656,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-5548212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-570400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-5713631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-6214550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-6228646,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-632744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-6425097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-6446553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-6452486,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-6452487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-666832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-6781489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-6809742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-6874690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-6874691,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-7082297,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-7202842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-7240256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2961324-7247411
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
246
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
745-53
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:2961324-6-Aminonicotinamide,
pubmed-meshheading:2961324-Abnormalities, Drug-Induced,
pubmed-meshheading:2961324-Animals,
pubmed-meshheading:2961324-Carbohydrates,
pubmed-meshheading:2961324-Centrifugation, Density Gradient,
pubmed-meshheading:2961324-Chick Embryo,
pubmed-meshheading:2961324-Chromatography, Gel,
pubmed-meshheading:2961324-Extremities,
pubmed-meshheading:2961324-Glucosamine,
pubmed-meshheading:2961324-Glycosides,
pubmed-meshheading:2961324-Limb Deformities, Congenital,
pubmed-meshheading:2961324-Niacinamide,
pubmed-meshheading:2961324-Proteoglycans,
pubmed-meshheading:2961324-Sulfates
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pubmed:year |
1987
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pubmed:articleTitle |
Altered proteoglycan synthesis by micromelial limbs induced by 6-aminonicotinamide. Appearance of abnormal forms of cartilage-characteristic proteoglycan (PG-H).
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pubmed:affiliation |
Department of Biochemistry, Tokyo College of Pharmacy, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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