| pubmed-article:2951440 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2951440 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:2951440 | lifeskim:mentions | umls-concept:C0039194 | lld:lifeskim |
| pubmed-article:2951440 | lifeskim:mentions | umls-concept:C0002199 | lld:lifeskim |
| pubmed-article:2951440 | lifeskim:mentions | umls-concept:C1704675 | lld:lifeskim |
| pubmed-article:2951440 | lifeskim:mentions | umls-concept:C0003261 | lld:lifeskim |
| pubmed-article:2951440 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:2951440 | lifeskim:mentions | umls-concept:C2349975 | lld:lifeskim |
| pubmed-article:2951440 | lifeskim:mentions | umls-concept:C0127400 | lld:lifeskim |
| pubmed-article:2951440 | lifeskim:mentions | umls-concept:C1627358 | lld:lifeskim |
| pubmed-article:2951440 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:2951440 | pubmed:dateCreated | 1987-5-6 | lld:pubmed |
| pubmed-article:2951440 | pubmed:abstractText | This study describes the effects of human recombinant IFN-alpha 2 on antibody production in vitro. Whereas the inclusion of IFN-alpha 2 in cultures for 7 days had a relatively minor effect on pokeweed mitogen (PWM)-induced antibody production, it resulted in a dose-related enhancement of a hapten-specific primary antibody response. Comparison of PWM and IFN-induced [3H]thymidine uptake indicated that the observed IFN activation was not polyclonal. Pretreatment of T cells with IFN for 1 hr before recombination with untreated autologous B lymphocytes increased the anti-TNP response four-fold, whereas similar pretreatment of B lymphocytes had no effect. Furthermore, 2000 R x-irradiation of T cells before coculture with autologous B lymphocytes and IFN abrogated the TNP-specific response. These results indicate that IFN modulates TNP-specific antibody production via a radiosensitive T-helper function. Further subfractionation by panning suggests that the enhancement is mediated by the Leu-3a+ helper/inducer T cell subset. Evidence that a 1-hr exposure to IFN was sufficient to modulate antibody production prompted the examination of T cells for possible receptor mechanisms. Scatchard analysis of 125I-IFN-alpha 2 binding revealed approximately 65 high affinity IFN receptors per cell with an apparent dissociation constant (Kd) of 4.4 X 10(-10) M. This paper is the first demonstration of the role of T cells in mediating the effects of recombinant IFN-alpha 2 on human primary antibody responses in vitro. These data further suggest that the observed modulation of hapten-specific antibody production in vitro by IFN may involve the binding of IFN to specific cellular receptors expressed by T lymphocytes. | lld:pubmed |
| pubmed-article:2951440 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2951440 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2951440 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2951440 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2951440 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:2951440 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2951440 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2951440 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2951440 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2951440 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:2951440 | pubmed:issn | 0022-1767 | lld:pubmed |
| pubmed-article:2951440 | pubmed:author | pubmed-author:OzerHH | lld:pubmed |
| pubmed-article:2951440 | pubmed:author | pubmed-author:EvansS SSS | lld:pubmed |
| pubmed-article:2951440 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2951440 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:2951440 | pubmed:volume | 138 | lld:pubmed |
| pubmed-article:2951440 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2951440 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2951440 | pubmed:pagination | 2451-6 | lld:pubmed |
| pubmed-article:2951440 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:2951440 | pubmed:meshHeading | pubmed-meshheading:2951440-... | lld:pubmed |
| pubmed-article:2951440 | pubmed:meshHeading | pubmed-meshheading:2951440-... | lld:pubmed |
| pubmed-article:2951440 | pubmed:meshHeading | pubmed-meshheading:2951440-... | lld:pubmed |
| pubmed-article:2951440 | pubmed:meshHeading | pubmed-meshheading:2951440-... | lld:pubmed |
| pubmed-article:2951440 | pubmed:meshHeading | pubmed-meshheading:2951440-... | lld:pubmed |
| pubmed-article:2951440 | pubmed:meshHeading | pubmed-meshheading:2951440-... | lld:pubmed |
| pubmed-article:2951440 | pubmed:meshHeading | pubmed-meshheading:2951440-... | lld:pubmed |
| pubmed-article:2951440 | pubmed:meshHeading | pubmed-meshheading:2951440-... | lld:pubmed |
| pubmed-article:2951440 | pubmed:meshHeading | pubmed-meshheading:2951440-... | lld:pubmed |
| pubmed-article:2951440 | pubmed:meshHeading | pubmed-meshheading:2951440-... | lld:pubmed |
| pubmed-article:2951440 | pubmed:meshHeading | pubmed-meshheading:2951440-... | lld:pubmed |
| pubmed-article:2951440 | pubmed:year | 1987 | lld:pubmed |
| pubmed-article:2951440 | pubmed:articleTitle | Enhancement of a human antibody response in vitro mediated by interaction of interferon-alpha with T lymphocytes. | lld:pubmed |
| pubmed-article:2951440 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2951440 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:2951440 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2951440 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
