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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1978-10-27
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pubmed:abstractText |
Dimeric creatine kinase (EC 2.7.3.2) from rabbit skeletal muscle can be immobilized via a single subunit to CNBr-activated Sepharose 4B and subsequently treated with guanidine hydrochloride followed by renaturation to yield a catalytically active matrix-bound subunit derivative. The importance of the intact dimeric structure in the activation of the enzyme by acetate was demonstrated. Immobilization did not appear to alter the pH optimum of the enzyme, and the kinetic parameters fot the matrix-bound derivatives were generally similar to those for the soluble enzyme, but the matrix-bound derivatives showed higher thermal stability and greater resistance to denaturation than did the soluble enzyme. The rates of reaction of thiol groups of the matrix-bound derivatives with iodoacetamide in the absence and in the presence of combinations of substrates were similar to those of the soluble enzyme. Studies with 5,5'-dithiobis-(2-nitrobenzoic acid) and with iodoacetamide revealed the presence of an additional reactive thiol group in the matrix-bound subunit derivative, which is presumably masked in the dimeric derivatives.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/28737-1022561,
http://linkedlifedata.com/resource/pubmed/commentcorrection/28737-1091485,
http://linkedlifedata.com/resource/pubmed/commentcorrection/28737-1276977,
http://linkedlifedata.com/resource/pubmed/commentcorrection/28737-1278386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/28737-13479429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/28737-13650640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/28737-13729393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/28737-14240539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/28737-170912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/28737-239687,
http://linkedlifedata.com/resource/pubmed/commentcorrection/28737-402942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/28737-4205557,
http://linkedlifedata.com/resource/pubmed/commentcorrection/28737-4356816,
http://linkedlifedata.com/resource/pubmed/commentcorrection/28737-4369851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/28737-4697391,
http://linkedlifedata.com/resource/pubmed/commentcorrection/28737-4735266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/28737-4850305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/28737-4856652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/28737-4882248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/28737-5129268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/28737-5530055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/28737-902910,
http://linkedlifedata.com/resource/pubmed/commentcorrection/28737-9068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/28737-962857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/28737-964263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/28737-986830
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
173
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
85-93
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:28737-Animals,
pubmed-meshheading:28737-Chemical Phenomena,
pubmed-meshheading:28737-Chemistry,
pubmed-meshheading:28737-Creatine Kinase,
pubmed-meshheading:28737-Dithionitrobenzoic Acid,
pubmed-meshheading:28737-Enzymes, Immobilized,
pubmed-meshheading:28737-Hydrogen-Ion Concentration,
pubmed-meshheading:28737-Iodoacetamide,
pubmed-meshheading:28737-Kinetics,
pubmed-meshheading:28737-Muscles,
pubmed-meshheading:28737-Proteins,
pubmed-meshheading:28737-Rabbits
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pubmed:year |
1978
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pubmed:articleTitle |
Properties of matrix-bound dimer and monomer derivatives of immobilized creatine kinase from rabbit skeletal muscle.
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pubmed:publicationType |
Journal Article
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