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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1988-12-22
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pubmed:abstractText |
Broad bean (Vicia faba) mitochondrial DNA (mtDNA) includes three circular plasmids: mt-plasmid 1 (1,704 ntp), mt-plasmid 2 (1,695 ntp) and mt-plasmid 3 (1,476 ntp). Partially replicated circular forms of these mt-plasmids have been observed in electron microscope preparations. Restriction enzymes that cleave either mt-plasmid 2 (but not mt-plasmids 1 and 3) or mt-plasmid 3 (but not mt-plasmids 1 and 2) were used to generate linear forms of partially replicated mt-plasmid 2 and mt-plasmid 3 molecules. Analyses of these linearized replicative intermediates, observed by electron microscopy, indicated that in both mt-plasmid 2 and mt-plasmid 3 replication originates at a specific location and proceeds in the same, single direction around the molecules. The replication origins of mt-plasmid 2 and mt-plasmid 3 map close to sequences that can fold into hairpin structures.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CCCGGG-specific type II...,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Circular,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Mitochondrial,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Restriction Enzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyribonucleases, Type II...
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0172-8083
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
163-70
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pubmed:dateRevised |
2008-8-29
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pubmed:meshHeading |
pubmed-meshheading:2846190-DNA, Circular,
pubmed-meshheading:2846190-DNA, Mitochondrial,
pubmed-meshheading:2846190-DNA Replication,
pubmed-meshheading:2846190-DNA Restriction Enzymes,
pubmed-meshheading:2846190-Deoxyribonucleases, Type II Site-Specific,
pubmed-meshheading:2846190-Fabaceae,
pubmed-meshheading:2846190-Microscopy, Electron,
pubmed-meshheading:2846190-Plants, Medicinal,
pubmed-meshheading:2846190-Plasmids
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pubmed:year |
1988
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pubmed:articleTitle |
Origin and direction of replication in mitochondrial plasmid DNAs of broad bean, Vicia faba.
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pubmed:affiliation |
Department of Biology, University of Utah, Salt Lake City 84112.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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