Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
1987-12-23
pubmed:abstractText
Mutants of Abelson virus encoding temperature-sensitive protein-tyrosine kinase (EC 2.7.1.112) were created by site-directed mutagenesis using sequence information from temperature-sensitive mutants of the related v-src oncogene. Expression of these two independent mutations in Escherichia coli resulted in reduced phosphorylation of the mutant proteins at high temperature. Viruses containing one of the mutations induced conditional transformation of both NIH 3T3 and lymphoid cells when expressed in the context of a truncated transforming protein. These results underscore the functional homology between protein-tyrosine kinases and suggest that transfer of mutations within a related gene family may provide a rapid method to create mutants.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-194709, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-209468, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-211510, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-222468, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-2416464, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-2434953, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-271968, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-2869410, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-2882603, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-2981357, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-2983108, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-2999430, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-3007997, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-3009882, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-3020782, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-3036494, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-3037315, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-3099169, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-3526330, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-3881765, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-3945311, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-4069211, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-4311641, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-4317808, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-4331732, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-503218, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-6096101, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-6211393, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-6253663, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-6254050, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-6257397, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-6257398, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-6257926, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-6269755, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-6278718, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-6283119, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-6291784, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-6292177, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-6298790, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-6300444, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-6300457, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-6304726, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-6313229, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-6316124, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-6351725, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-6449003, http://linkedlifedata.com/resource/pubmed/commentcorrection/2825174-6954007
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:volume
84
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
8021-5
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1987
pubmed:articleTitle
Isolation of temperature-sensitive Abelson virus mutants by site-directed mutagenesis.
pubmed:affiliation
Department of Molecular Biology, Tufts University School of Medicine, Boston, MA 02111.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.